| 產品編號 | bs-13201R |
| 英文名稱 | Rabbit Anti-FOXF2 antibody |
| 中文名稱 | 叉頭蛋白F2抗體 |
| 別 名 | FKH L6; FKHL 6; FKHL6; Forkhead box F2; Forkhead box protein F2; Forkhead like 6; Forkhead related activator 2; Forkhead related protein FKHL6; Forkhead related transcription factor 2; FOX F2; FOXF 2; FREAC 2; FREAC2; OTTHUMP00000017863; |
| 研究領域 | 腫瘤 細胞生物 發育生物學 神經生物學 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Chicken) |
| 產品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FOXF2: 65-160/444 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The FOX family of transcription factors share a common DIUA binding domain termed a winged-helix or forkhead domain. Many FOX proteins play important roles in development, metabolism, cancer and aging. Development of the vertebrate gut is controlled by paracrine crosstalk between the endodermal epithelium and the associated splanchnic mesoderm. FOXF2 (forkhead box F2), also known as FKHL or FREAC2, is expressed in lung and placenta, and has been shown to transcriptionally activate several lung-specific genes. FOXF2 interacts with transcription factors TFIIB and TBP, and may be involved in regulating transcription in embryogenesis and pattern formation in multicellular organisms. FOXF2-deficient mice develop cleft palate and an abnormal tongue, which suggests that FOXF2 may be critical for palatogenesis. Function: FOXF2 encodes forkhead box F2, one of many human homologues of the Drosophila melanogaster transcription factor forkhead. FOXF2 is expressed in lung and placenta, and has been shown to transcriptionally activate several lung-specific genes. Subunit: Interacts with the transcription factors TBP and TFIIB. Subcellular Location: Nuclear. Similarity: Contains 1 fork-head DNA-binding domain. SWISS: Q12947 Gene ID: 2295 Database links: Entrez Gene: 2295 Human Entrez Gene: 14238 Mouse Omim: 603250 Human SwissProt: Q12947 Human SwissProt: O54743 Mouse Unigene: 484423 Human Unigene: 125548 Mouse |
| 產品圖片 |
ample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Lung tissue lysates
Primary: Anti-FOXF2 (bs-13201R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- FOXF2 (bs-13201R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
Sample:
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti- FOXF2 (bs-13201R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
