| 產品編號 | bs-12494R |
| 英文名稱 | Rabbit Anti-APOBEC3B antibody |
| 中文名稱 | 載脂蛋白B-mRNA編輯酶復合物3B抗體 |
| 別 名 | APOBEC1L; Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3B; apolipoprotein B mRNA editing enzyme, catalytic;ARCD3; ARP4; bK150C2.2; Cytidine deaminase; DJ742C19.2; FLJ21201; Phorbolin 1 related protein; Phorbolin 2; Phorbolin 2/3; Phorbolin 3; PHRBNL; Probable DNA dC>dU editing enzyme APOBEC3B. |
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Specific References (1) | bs-12494R has been referenced in 1 publications.
[IF=2.02] Onguru, Onder, et al. "APOBEC3B expression in drug resistant MCF-7 breast cancer cell lines." Biomedicine & Pharmacotherapy 79 (2016): 87-92. WB, IHC-P ; Human.
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| 研究領域 | 細胞生物 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human APOBEC3B: 1-100/382 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
APOBEC proteins inhibit retroviruses by deaminating cytosine residues of viral RNA and DNA. The seven APOBEC3 genes or pseudogenes are found in a cluster thought to result from gene duplication on chromosome 22. APOBEC3 proteins are thought to be RNA editing enzymes and have roles in cell growth. APOBEC3B, also known as phorbolin-1-related protein or phorbolin-2/3, is a 382 amino acid protein belonging to the cytidine and deoxycytidylate deaminase family. APOBEC3B lacks cytidine deaminase activity on RNA molecules, but has been shown to bind to apoB and AU-rich RNAs. APOBEC3B forms a homodimer and interacts with APOBEC3G. APOBEC3B is expressed at at moderate levels in heart, testis, thymus, prostate, ovary, spleen and peripheral blood leukocytes. Function: APOBEC3B is a member of the cytidine deaminase gene family. However, it lacks cytidine deaminase activity, at least on RNA molecules (monomeric nucleoside substrates or synthetic apoB, NF1 and NAT1 RNA template). It binds to apoB and AU-rich RNAs. It is unable to reduce HIV-1 infectivity in vitro. Subunit: Homodimer. Interacts with APOBEC3G. Does not interact with APOBEC1. Subcellular Location: Nucleus. Tissue Specificity: Expressed at high and moderate levels in peripheral blood leukocytes, spleen, testes, heart, thymus, prostate and ovary. Also expressed at low levels in several other tissues. Similarity: It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Belongs to the cytidine and deoxycytidylate deaminase family. Contains 2 CMP/dCMP deaminase zinc-binding domains SWISS: Q9UH17 Gene ID: 9582 Database links: Entrez Gene: 9582 Human Omim: 607110 Human SwissProt: Q9UH17 Human Unigene: 226307 Human Unigene: 658626 Human Unigene: 29180 Rat |
| 產品圖片 |
Sample:jurkat(human)cell Lysate at 40 ug
Primary: Anti-APOBEC3B (bs-12494R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46kD
Observed band size: 46 kD
Tissue/cell: Rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated(bs-12494R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated(bs-12494R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated(bs-12494R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
