| 產品編號 | bs-7416R |
| 英文名稱 | Rabbit Anti-DNA polymerase delta antibody |
| 中文名稱 | DNA聚合酶δ 抗體 |
| 別 名 | DNA polymerase delta subunit 2; DNA polymerase delta subunit p50; DNA polymerase subunit delta 2; DNA polymerase subunit delta p50; DPOD2_HUMAN; POLD 2; POLD2. |
| 研究領域 | 細胞生物 染色質和核信號 信號轉導 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Human DNA polymerase delta: 101-200/469 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases (1). In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches (2). These exonucleases include the family of DNA polymerases (3). DNA pol α, β, ?, and e are involved in DNA replication and repair (4). DNA pol ? and DNA pol e are multisubunit enzymes, with DNA pol ? consisting of two subunits p125, which interacts with the sliding DNA clamp protein PCNA, and p50 (5). The nuclear-encoded DNA pol ? is the only DNA polymerase required for the replication of the mitochondrial DNA (6). DNA pol Ω is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis (7). DNA pol ? is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks (8).
Function: The function of the small subunit is not yet clear. Subcellular Location: Nucleus. Similarity: Belongs to the DNA polymerase delta/II small subunit family. SWISS: P49005 Gene ID: 5425 Database links: Entrez Gene: 5425 Human Entrez Gene: 18972 Mouse Omim: 600815 Human SwissProt: P49005 Human SwissProt: O35654 Mouse Unigene: 306791 Human Unigene: 35788 Mouse Unigene: 22989 Rat |
| 產品圖片 |
Sample:
MOLT-4(Human) Cell Lysate at 30 ug
Primary: Anti-DNA polymerase delta (bs-7416R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-DNA polymerase delta (bs-7416R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Lane 1: Mouse Skin tissue lysates
Lane 2: Mouse Large intestine tissue lysates
Lane 3: Mouse Testis tissue lysates
Primary: Anti-DNA polymerase delta (bs-7416R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (POLD2) Polyclonal Antibody, Unconjugated (bs-7416R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
