| 產品編號 | bs-11913R |
| 英文名稱 | Rabbit Anti-NPDC1 antibody |
| 中文名稱 | 神經細胞增殖和分化調控蛋白1抗體 |
| 別 名 | AI314472; CAB1; Neural proliferation differentiation and control protein 1; NPDC-1; NPDC1; NPDC1_HUMAN. |
| 研究領域 | 神經生物學 細胞凋亡 細胞周期蛋白 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NPDC1: 75-180/325 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
NPDC-1 (Neural Proliferation Differentiation and Control-1) is expressed in neurons once they have stopped dividing and begun to differentiate. NPDC-1 is transported from the Golgi apparatus via vesicles before becoming internalized by endosomes at the cell membrane. NPDC-1 interacts with Cdk2, D-type cyclins, and the transcription factor E2F1. This interaction can lead to an increased replication time, and might have implications in final neural differentiation and apoptosis. NPDC-1 has been shown to colocalize with synaptic vesicle proteins: synaptophysin, synaptobrevin 2, and Rab3 GEP (Rab3 GTP/GDP exchange protein). One function of NPDC-1 is to regulate retinoic acid-mediated events by directly interacting with retinoid receptors. The amino acid sequence of NPDC-1 is highly conserved between mouse, rat, and human. Function: Suppresses oncogenic transformation in neural and non-neural cells and down-regulates neural cell proliferation. Might be involved in transcriptional regulation. Subcellular Location: Membrane; Single-pass membrane protein (Potential). Tissue Specificity: Strongly expressed in adult brain; especially in hippocampus, frontal lobe and temporal lobe. Similarity: Belongs to the NPDC1/cab-1 family. SWISS: Q9NQX5 Gene ID: 56654 Database links: Entrez Gene: 56654 Human Entrez Gene: 18146 Mouse Omim: 605798 Human SwissProt: Q9NQX5 Human SwissProt: Q64322 Mouse Unigene: 719906 Human Unigene: 1131 Mouse Unigene: 5802 Rat |
| 產品圖片 |
Sample: Uterus (mouse) Lysate at 40 ug
Primary: Anti- NPDC1(bs-11913R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Sample: Large intestine (mouse) Lysate at 40 ug
Primary: Anti- NPDC1(bs-11913R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
