| 產(chǎn)品編號 | bs-11768R |
| 英文名稱 | Rabbit Anti-TDRD3 antibody |
| 中文名稱 | Tudor結構域蛋白TDRD3抗體 |
| 別 名 | TDRD 3; tdrd3; TDRD3_HUMAN; Tudor domain containing 3; Tudor domain containing protein 3; Tudor domain-containing protein 3; 4732418C03Rik; 6720468N18; FLJ21007; OTTHUMP00000018485. |
| 研究領域 | 細胞生物 神經(jīng)生物學 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Chicken,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 73kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TDRD3: 161-250/651 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Tudor domain containing 3 is a 651 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one UBA domain and one tudor domain. Expressed in lung, brain, heart, liver, placenta, kidney, pancreas and skeletal muscle, TDRD3 exists as a component of mRNA stress granules and is thought to play a role in the translation of target mRNAs, as well as in the assembly and disassembly of stress granules. Multiple isoforms of TDRD3 exist due to alternative splicing events. The gene encoding TDRD3 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. Trisomy 13, also known as Patau syndrome, is deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections. Function: Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins. Subunit: Component of mRNA stress granules. Interacts with FMR1, FXR1, FXR2, EWSR1, FUS, SERBP1, EEF1A1 and DDX3X or DDX3Y, and with the small nuclear ribonucleoprotein-associated proteins SNRPB and SNRPN. Interacts with 'Lys-48'-linked tetra-ubiquitin, but not with monoubiquitin or 'Lys-63'-linked ubiquitin chains. Subcellular Location: Cytoplasm. Nucleus. Predominantly cytoplasmic. Associated with actively translating polyribosomes and with mRNA stress granules. Tissue Specificity: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Similarity: Contains 1 Tudor domain. Contains 1 UBA domain. SWISS: Q9H7E2 Gene ID: 81550 Database links: Entrez Gene: 418823 Chicken Entrez Gene: 452750 Chimpanzee Entrez Gene: 81550 Human Entrez Gene: 219249 Mouse Entrez Gene: 431810 Xenopus laevis Entrez Gene: 395019 Xenopus tropicalis Omim: 614392 Human SwissProt: Q5ZMS6 Chicken SwissProt: Q9H7E2 Human SwissProt: Q91W18 Mouse SwissProt: Q6NRP6 Xenopus laevis SwissProt: Q6P1U3 Xenopus tropicalis |
| 產(chǎn)品圖片 |
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti- TDRD3 (bs-11768R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73kD
Observed band size: 73kD
Sample:
pc-3 Cell (Human) Lysate at 30 ug
Primary: Anti-TDRD3 (bs-11768R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kD
Observed band size: 77 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TDRD3) Polyclonal Antibody, Unconjugated (bs-11768R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
