| 產品編號 | bs-9766R |
| 英文名稱 | Rabbit Anti-INTS3 antibody |
| 中文名稱 | 集成復雜蛋白亞單位3抗體 |
| 別 名 | Integrator complex subunit 3; C1orf193; C1orf60; FLJ21919; INT 3; INT3; INTS3; SOSS complex subunit A. |
| 研究領域 | 細胞生物 信號轉導 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Rabbit,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 118kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human INTS3: 901-1043/1043 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The integrator complex is a multi-protein complex that associates with the C-terminal domain of Pol II and is involved in small nuclear RNA (snRNA) transcription and 3’-end processing. INTS3 (integrator complex subunit 3), also known as INT3 or SOSS-A, is a 1,043 amino acid nuclear protein and is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit and mediates 3-prime end processing of small nuclear RNAs U1 and U2. Expressed in the nucleus, INTS3 exists as four alternatively spliced isoforms and is encoded by a gene located on human chromosome 1q21.3. Subunit: Belongs to the multiprotein complex Integrator, at least composed of INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, CPSF3L/INTS11 and INTS12. Component of the SOSS complex, composed of SOSS-B (SOSS-B1/OBFC2B or SOSS-B2/OBFC2A), SOSS-A/INTS3 and SOSS-C/C9orf80. SOSS complexes containing SOSS-B1/OBFC2B are more abundant than complexes containing SOSS-B2/OBFC2A. Interacts with SOSS-B1/OBFC2B, SOSS-B2/OBFC2A and SOSS-C/C9orf80; the interaction is direct. Interacts with NBN/NBS1. Subcellular Location: Nucleus. Note=Localizes to nuclear foci following DNA damage. Similarity: Belongs to the Integrator subunit 3 family. SWISS: Q68E01 Gene ID: 65123 Database links: Entrez Gene: 65123 Human Entrez Gene: 229543 Mouse Omim: 611347 Human SwissProt: Q68E01 Human SwissProt: Q7TPD0 Mouse |
| 產品圖片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Primary: Anti- INTS3 (bs-9766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kD
Observed band size: 118 kD
Paraformaldehyde-fixed, paraffin embedded (Human uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (INTS3) Polyclonal Antibody, Unconjugated (bs-9766R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (INTS3) Polyclonal Antibody, Unconjugated (bs-9766R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
