| 產品編號 | bs-9337R |
| 英文名稱 | Rabbit Anti-MARCH3 antibody |
| 中文名稱 | 膜相關環指蛋白3抗體 |
| 別 名 | E3 ubiquitin-protein ligase MARCH3; FLJ20096; MARCH-III; March3; MARH3_HUMAN; Membrane associated ring finger (C3HC4) 3; Membrane-associated RING finger protein 3; Membrane-associated RING-CH protein III; RING finger protein 173; RNF173. |
| 研究領域 | 細胞生物 免疫學 信號轉導 通道蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 29kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MARCH3: 101-200/253 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). MARCH3 (membrane-associated ring finger (C3HC4) 3), also known as RNF173, is a 253 amino acid multi-pass membrane protein that localizes to cytoplasmic vesicles and early endosomes and contains one RING-CH-type zinc finger. Involved in the pathway of protein modification, MARCH3 functions as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and is thought to be involved in endosomal trafficking events. Function: E3 ubiquitin-protein ligase which may be involved in endosomal trafficking. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates (By similarity). Subunit: Interacts with MARCH2 and STX6 (By similarity). Subcellular Location: Cytoplasmic vesicle membrane. Early endosome membrane. Similarity: Contains 1 RING-CH-type zinc finger. SWISS: Q86UD3 Gene ID: 115123 Database links: Entrez Gene: 115123 Human Entrez Gene: 320253 Mouse Omim: 613333 Human SwissProt: Q86UD3 Human SwissProt: Q8BRX9 Mouse Unigene: 132441 Human Unigene: 312623 Mouse Unigene: 203312 Rat |
| 產品圖片 |
Sample:
Eye (Mouse) Lysate at 40 ug
Primary: Anti- MARCH3 (bs-9337R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
