| 產品編號 | bs-8383R |
| 英文名稱 | Rabbit Anti-UBE2Q1 antibody |
| 中文名稱 | 泛素蛋白連接酶Q1抗體 |
| 別 名 | Galactosyl transferase associated protein; GTAP; NICE 5; NICE5; PRO3094; Protein NICE 5; Protein NICE-5; UB2Q1_HUMAN; UBE2Q; Ube2q1; Ubiquitin carrier protein Q1; Ubiquitin conjugating enzyme E2 Q1; Ubiquitin conjugating enzyme E2Q (putative) 1; Ubiquitin conjugating enzyme E2Q 1; Ubiquitin conjugating enzyme E2Q family member 1; Ubiquitin protein ligase Q1; Ubiquitin-conjugating enzyme E2 Q1; Ubiquitin-protein ligase Q1. |
| 研究領域 | 細胞生物 免疫學 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Sheep,Cow,Dog,Horse) |
| 產品應用 | Flow-Cyt=2μg /Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human UBE2Q1: 321-422/422 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (UBE1s), ubiquitin-conjugating enzymes (UBE2s), and ubiquitin-protein ligases (UBE3s). When ubiquitin is activated by a UBE1, it is transferred to the cysteine residue on a UBE2. UBE2 then binds a UBE3, which transfers the ubiquitin from the UBE2 cysteine to a lysine residue on the target protein. Ubiquitin-conjugating enzyme E2 Q1 (UBE2Q1), also known as ubiquitin-protein ligase Q1 or ubiquitin carrier protein Q1, is an 422 amino acid protein belonging to the ubiquitin-conjugating enzyme (UBE2) family. Two named isoforms of UBE2Q1 exist as a result of alternative splicing events.
Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Tissue Specificity: Widely expressed. Similarity: Belongs to the ubiquitin-conjugating enzyme family. SWISS: Q7Z7E8 Gene ID: 55585 Database links: UniProtKB/Swiss-Prot: Q7Z7E8.1 |
| 產品圖片 |
cells:mouse splenocyte
Concentration: 2μg/10^6 cells, 4℃ incubate 30min.
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-UBE2Q1 antibody (bs-8383R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
