| 產品編號 | bs-8334R |
| 英文名稱 | Rabbit Anti-NIMP/RTN4IP1 antibody |
| 中文名稱 | NOGO相互作用線粒體蛋白1抗體 |
| 別 名 | mitochondrial; NIMP; NOGO interacting mitochondrial protein; NOGO-interacting mitochondrial protein; Reticulon 4 interacting protein 1; Reticulon 4 interacting protein 1, mitochondrial; Reticulon-4-interacting protein 1; RT4I1_HUMAN; Rtn4ip1; NIMP/RTN4IP1. |
| 研究領域 | 腫瘤 細胞生物 神經生物學 信號轉導 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig) |
| 產品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39kDa |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NIMP/RTN4IP1: 111-210/396 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Appears to be a potent inhibitor of regeneration following spinal cord injury.Nogo is an oligodendrocyte-specific member of the Reticulon family and is a component of CNS white matter that inhibits axon outgrowth, induces collapse of growth cones of chick dorsal root ganglion cells, and inhibits the spreading of 3T3 fibroblasts. Nogo is expressed by oligodendrocytes but not by Schwann cells, and associates primarily with the endoplasmic reticulum. Nogo exists in three different splice forms, Nogo-A, -B and -C. NIMP (NOGO-interacting mitochondrial protein), also known as RTN4IP1 (Reticulon-4-interacting protein 1), is a 396 amino acid mitochondrial protein that contains a C-terminal oxidoreductaselike domain and numerous sites for phosphorylation. NIMP is expressed in mitochondrial-rich tissue such as kidney, heart, skeletal muscle and specific regions within the nervous system. Through interaction with Nogo, it is likely that NIMP plays a role in Nogo-induced inhibition of neurite growth. There are three isoforms of NIMP that are produced as a result of alternative splicing events. Function: Appears to be a potent inhibitor of regeneration following spinal cord injury. Subunit: Interacts with RTN4, UQCRC1 and UQCRC2 (By similarity). Subcellular Location: Mitochondrion. Tissue Specificity: Widely expressed in mitochondria-enriched tissues. Found in heart, muscle, kidney, liver, brain and placenta. Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. SWISS: Q8WWV3 Gene ID: 84816 Database links: Entrez Gene: 84816 Human Entrez Gene: 170728 Mouse Omim: 610502 Human SwissProt: Q8WWV3 Human SwissProt: Q924D0 Mouse Unigene: 155839 Human Unigene: 390253 Mouse Unigene: 220185 Rat |
| 產品圖片 |
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- NIMP/RTN4IP1 (bs-8334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 40 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- NIMP/RTN4IP1 (bs-8334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
