| 產品編號 | bs-7770R |
| 英文名稱 | Rabbit Anti-SEPT10 antibody |
| 中文名稱 | 細胞分化蛋白SEPT10抗體 |
| 別 名 | SEPT 10; SEPT-10; Septin 10; Septin-10; Septin 10; SEP10_HUMAN. |
| 研究領域 | 細胞生物 細胞周期蛋白 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Chicken,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SEPT10: 121-220/454 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The SEPT10 gene encodes a member of the septin family of cytoskeletal proteins which have GTPase activity. This protein localizes to the cytoplasm and nucleus and displays GTP-binding and GTPase activity. Alternate splicing results in two transcript variants encoding different isoforms. Function: Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). Subunit: Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation (By similarity). Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton (By similarity). Note=Using a GFP-fusion protein, detected in the nucleus (PubMed:12711328). Tissue Specificity: Widely expressed. Abundantly expressed in heart and kidney, placenta, skeletal muscles, liver and lung, as well as various tumor cell lines. Similarity: Belongs to the septin family. SWISS: Q9P0V9 Gene ID: 151011 Database links: Entrez Gene: 151011 Human Entrez Gene: 103080 Mouse Omim: 611737 Human SwissProt: Q9P0V9 Human SwissProt: Q8C650 Mouse Unigene: 469615 Human Unigene: 346842 Mouse Unigene: 397041 Mouse SEPTIN(SEPT)家族是細胞骨架蛋白的一種，具有GTPase的活性。經研究發現，SEPT參與細胞核分裂、細胞膜的運輸以及構成細胞骨架。 |
| 產品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- SEPT10 (bs-7770R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
