| 產品編號 | bs-7734R |
| 英文名稱 | Rabbit Anti-CAPD3 antibody |
| 中文名稱 | 濃縮素2復合亞基D3抗體 |
| 別 名 | CAP D3; CAPD 3; CAPD3; Condensin II complex subunit D3; Hcp 6; Hcp6; hHCP 6; hHCP6; NCAPD 3; NCAPD3; Non SMC condensin II complex subunit D3; CNDD3_HUMAN. |
| 研究領域 | 細胞生物 細胞周期蛋白 細胞分化 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 169kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CAPD3/hCAP-D3: 651-760/1498 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4. hCAP-D3 is a regulatory non-SMC subunit of the condensin II complex. Function: Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis. Subunit: Component of the condensin-2 complex, which contains the SMC2 and SMC4 heterodimer, and 3 non SMC subunits that probably regulate the complex: NCAPH2, NCAPD3 and NCAPG2. Subcellular Location: Nucleus. Similarity: Contains 4 HEAT repeats. SWISS: P42695 Gene ID: 23310 Database links: Entrez Gene: 23310 Human Omim: 609276 Human SwissProt: P42695 Human Unigene: 438550 Human |
| 產品圖片 |
Blank control:RSC96 (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- CAPD3 antibody(bs-7734R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
