| 產品編號 | bs-6818R |
| 英文名稱 | Rabbit Anti-MAGEA12 antibody |
| 中文名稱 | 黑色素瘤相關抗原12抗體 |
| 別 名 | Cancer/testis antigen 1.12; Cancer/testis antigen family 1 member 12; CT1.12; MAGAC_HUMAN; MAGE 12 antigen; MAGE-12 antigen; MAGE12; MAGE12 antigen; MAGE12F; MAGE12F antigen; MAGEA12; Melanoma antigen family A 12; Melanoma associated antigen 12; Melanoma-associated antigen 12. |
| 研究領域 | 腫瘤 細胞生物 信號轉導 細胞類型標志物 腫瘤細胞生物標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAGEA12: 121-210/314 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Not known, though may play a role in tumor transformation or aspects of tumor progression.
Tissue specificity: Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Function: Not known, though may play a role in tumor transformation or aspects of tumor progression. Tissue Specificity: Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Similarity: Contains 1 MAGE domain. SWISS: P43365 Gene ID: 4111 Database links: Entrez Gene: 4111 Human Omim: 300177 Human SwissProt: P43365 Human Unigene: 169246 Human |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAGEA12) Polyclonal Antibody, Unconjugated (bs-6818R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
