| 產品編號 | bs-6817R |
| 英文名稱 | Rabbit Anti-MAGEA11 antibody |
| 中文名稱 | 黑色素瘤相關抗原11抗體 |
| 別 名 | Cancer/testis antigen 1.11; cancer/testis antigen family 1, member 11; CT1.11; MAGE 11; MAGE 11 antigen; MAGAB_HUMAN; MAGE11; MAGEA 11; Melanoma antigen family A 11; Melanoma associated antigen 11; MGC10511. |
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Specific References (1) | bs-6817R has been referenced in 1 publications.
[IF=1.89] Choudhary, Meenakshi, et al. "Enhancing Lung Cancer Diagnosis: Electrochemical Simultaneous Bianalyte Immunosensing Using Carbon Nanotubes–Chitosan Nanocomposite." Applied Biochemistry and Biotechnology (2014): 1-13. other ;
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| 研究領域 | 腫瘤 細胞生物 免疫學 染色質和核信號 信號轉導 腫瘤細胞生物標志物 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAGEA11: 12-120/429 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
MAGEA11 (Melanoma antigen family A, 11) is a member of the MAGEA gene family. MAGEA proteins are expressed in melanomas and other cancers. MAGEA11 acts as androgen receptor co-regulator increasing androgen receptor activity by modulating the receptors interdomain interaction. Function: Acts as androgen receptor co-regulator that increases androgen receptor activity by modulating the receptors interdomain interaction. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Subcellular Location: Nucleus. Cytoplasm. Tissue Specificity: Expressed in tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma. Expressed in testis, ovary, prostate, cancerous prostate, breast and adrenal tissue. Similarity: Contains 1 MAGE domain. SWISS: P43364 Gene ID: 4110 Database links: Entrez Gene: 4110 Human Omim: 300344 Human SwissProt: P43364 Human Unigene: 670252 Human |
| 產品圖片 |
Sample:
Lane 1: Human A431 cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human U-2 OS cell lysates
Primary: Anti-MAGEA11 (bs-6817R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 53 kDa
Sample:
Siha(Human) Cell Lysate at 30 ug
Primary: Anti-MAGEA11 (bs-6817R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (MAGEA11) Polyclonal Antibody, Unconjugated (bs-6817R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
