| 產品編號 | bs-6732R |
| 英文名稱 | Rabbit Anti-SDF4 antibody |
| 中文名稱 | 基質細胞衍生因子4抗體（鈣結合蛋白） |
| 別 名 | CAB45; Calcium binding protein; PSEC0034; SDF 4; SDF-4; Stromal cell derived factor 4; CAB45_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 信號轉導 生長因子和激素 結合蛋白 腫瘤細胞生物標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38kDa |
| 細胞定位 | 細胞漿 細胞膜 細胞外基質 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SDF4: 121-230/362 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a stromal cell derived factor that is a member of the CREC protein family. The encoded protein contains six EF-hand motifs and calcium-binding motifs. This protein localizes to the Golgi lumen and may be involved in regulating calcium dependent cellular activities. [provided by RefSeq, Sep 2011].
Function: May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment (By similarity). Isoform 5 may be involved in the exocytosis of zymogens by pancreatic acini. [COFACTOR] Binds calcium (By similarity). Isoform 5 binds calcium. Subunit: Isoform 5 interacts with STXBP1; the interaction is enhanced in presence of calcium. Isoform 5 interacts with STX3. Subcellular Location: Isoform 1: Golgi apparatus lumen (By similarity). Isoform 5: Cytoplasm. Cell projection, bleb. Note=Isoform 5 colocalizes with STX3 and STXBP1 isoform 2 at the plasma membrane and cell surface blebs. Tissue Specificity: Ubiquitous. Isoform 5 is expressed in pancreas. Similarity: Belongs to the CREC family. Contains 6 EF-hand domains. SWISS: Q9BRK5 Gene ID: 51150 Database links: Entrez Gene: 51150 Human Entrez Gene: 20318 Mouse Omim: 614282 Human SwissProt: Q9BRK5 Human SwissProt: Q61112 Mouse Unigene: 42806 Human Unigene: 293517 Mouse Unigene: 12964 Rat |
| 產品圖片 |
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human U87MG cell lysates
Lane 3: Human Jurkat cell lysates
Lane 4: Human U251 cell lysates
Primary: Anti-SDF4 (bs-6732R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 45 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SDF4) Polyclonal Antibody, Unconjugated (bs-6732R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
