| 產(chǎn)品編號 | bs-6645R |
| 英文名稱 | Rabbit Anti-WNT7A antibody |
| 中文名稱 | 原癌基因wnt7a蛋白抗體 |
| 別 名 | Protein Wnt-7a; wnt 7a;Protein Wnt-7a precursor; proto-oncogene wnt7a protein; wingless-type MMTV integration site family, member 7A; WNT7A; WNT7A_HUMAN. |
 |
Specific References (2) | bs-6645R has been referenced in 2 publications.
[IF=5.738] Jianglin Wang. et al. Validation and analysis of expression, prognosis and immune infiltration of WNT gene family in non-small cell lung cancer. FRONT ONCOL. 2022; 12: 911316 WB, IHC ; Human.
[IF=0.375] ZHANG P et al. 5-Azacytidine and trichostatin A enhance the osteogenic differentiation of bone marrow mesenchymal stem cells isolated from steroid-induced avascular necrosis of the femoral head in rabbit. J Biosci (2019) 44:87. WB ; Rabbit.
|
| 研究領域 | 腫瘤 信號轉(zhuǎn)導 干細胞 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41kDa |
| 細胞定位 | 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WNT7A: 241-349/349 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts. Function: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts (By similarity). Subunit: Interacts with PORCN. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain. DISEASE: Defects in WNT7A are the cause of limb pelvis hypoplasia aplasia syndrome (LPHAS) [MIM:276820]. A syndrome of severe deficiency of the extremities due to hypo- or aplasia of one or more long bones of one or more limbs. Pelvic manifestations include hip dislocation, hypoplastic iliac bone and aplastic pubic bones. Thoracic deformity, unusual facies and genitourinary anomalies can be present. Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS) [MIM:228930]; also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia. Similarity: Belongs to the Wnt family. SWISS: O00755 Gene ID: 7476 Database links: Entrez Gene: 7476 Human Entrez Gene: 100055450 Horse Entrez Gene: 22421 Mouse Entrez Gene: 100355697 Rabbit Omim: 601570 Human SwissProt: O00755 Human SwissProt: P24383 Mouse Unigene: 72290 Human Unigene: 56964 Mouse |
| 產(chǎn)品圖片 |
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-WNT7A (bs-6645R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 50 kD
Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Lung tissue lysates
Lane 3: Mouse Testis tissue lysates
Lane 4: Mouse Kidney tissue lysates
Lane 5: Rat Placenta tissue lysates
Lane 6: Rat Lung tissue lysates
Lane 7: Rat Testis tissue lysates
Lane 8: Rat Kidney tissue lysates
Lane 9: Human Hela cell lysates
Lane 10: Human A431 cell lysates
Lane 11: Human SH-SY5Y cell lysates
Lane 12: Human HepG2 cell lysates
Primary: Anti- WNT7A (bs-6645R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
