| 產品編號 | bs-7007R |
| 英文名稱 | Rabbit Anti-RNF43 antibody |
| 中文名稱 | 環指蛋白43抗體 |
| 別 名 | RNF124; Ring finger protein 43; URCC; RNF43_HUMAN; E3 ubiquitin-protein ligase RNF43; RING finger protein 43. |
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Specific References (1) | bs-7007R has been referenced in 1 publications.
[IF=8.071] Yue Wang. et al. CircRNA-IGLL1/miR-15a/RNF43 axis mediates ammonia-induced autophagy in broilers jejunum via Wnt/β-catenin pathway. Environ Pollut. 2022 Jan;292:118332 WB ; Chicken.
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| 研究領域 | 腫瘤 細胞生物 腫瘤細胞生物標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 83kDa |
| 細胞定位 | 細胞核 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RNF43: 41-140/783 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
RNF43 is a probable E3 ubiquitin-protein ligase that promotes cell growth and is upregulated in colon cancer. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Function: Probable E3 ubiquitin-protein ligase. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Subunit: Interacts with AKAP8L, NONO and SFPQ. Subcellular Location: Endoplasmic reticulum membrane; Single-pass type I membrane protein (Potential). Nucleus envelope. Tissue Specificity: Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines. Similarity: Contains 1 RING-type zinc finger. SWISS: Q68DV7 Gene ID: 54894 Database links: Entrez Gene: 54894 Human Entrez Gene: 207742 Mouse Omim: 612482 Human SwissProt: Q68DV7 Human SwissProt: Q5NCP0 Mouse Unigene: 584916 Human Unigene: 440230 Mouse Unigene: 214174 Rat |
| 產品圖片 |
Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human A549 cell lysates
Lane 4: Human HL-60 cell lysates
Primary: Anti-RNF43 (bs-7007R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 110 kDa
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RNF43 Polyclonal Antibody, Unconjugated(bs-7007R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
