| 產品編號 | bs-6554R |
| 英文名稱 | Rabbit Anti-NOL8 antibody |
| 中文名稱 | 核仁蛋白8抗體 |
| 別 名 | C9orf34; NOL8; NOL8_HUMAN; NOP132; Nucleolar protein 8; Nucleolar protein Nop132. |
| 研究領域 | 腫瘤 腫瘤細胞生物標志物 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 132kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NOL8: 801-900/1167 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Summary: NOL8 binds Ras-related GTP-binding proteins (see MIM 608267) and plays a role in cell growth
Function: Plays an essential role in the survival of diffuse-type gastric cancer cells. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells. Subunit: Interacts with the GTP form of RRAGA, RRAGC and RRAGD. Interacts with NIP7. Interacts with DDX18; the interaction is RNA-dependent. Interacts with DDX47; the interaction is RNA-dependent. Subcellular Location: Nucleus, nucleolus. Note=Localizes in the nucleolar-organizing region during ribosome biogenesis. Tissue Specificity: Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle. Post-translational modifications: Phosphorylated Similarity: Contains 1 RRM (RNA recognition motif) domain. SWISS: Q76FK4 Gene ID: 55035 Database links: Entrez Gene: 55035 Human Omim: 611534 Human SwissProt: Q76FK4 Human Unigene: 442199 Human |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NOL8) Polyclonal Antibody, Unconjugated (bs-6554R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
