| 產品編號 | bs-5748R |
| 英文名稱 | Rabbit Anti-DBF4B antibody |
| 中文名稱 | S期激酶活化蛋白DBF4B抗體 |
| 別 名 | Activator of S phase kinase like protein 1; ASK like protein 1; ASKL1; chifb; Chiffon homolog B; DBF 4B; DBF4 homolog B (S. cerevisiae); DBF4 homolog B; Dbf4 related factor 1; DRF1; Protein DBF4 homolog B; ZDBF1B; Zinc finger DBF type containing 1B; DBF4B_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 細胞周期蛋白 激酶和磷酸酶 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 18kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DBF4B/ASKL1: 41-140/615 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
DBF4B is a regulatory subunit for CDC7, a serine-threonine kinase which links cell cycle regulation to genome duplication. The complex CDC7-DBF4B selectively phosphorylates the MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. DBF4B localizes to the nucleus and its expression is cell cycle-regulated. Function: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. Subunit: Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4/DBF4A or DBF4B. Such complexes are stable upon replication stress. Subcellular Location: Nucleus. Note=Predominantly found in soluble fraction but not in the chromatin-bound fraction. Tissue Specificity: Widely expressed. Highly expressed in testis. Post-translational modifications: Phosphorylated. Similarity: Contains 1 BRCT domain. Contains 1 DBF4-type zinc finger. SWISS: Q8NFT6 Gene ID: 80174 Database links: Entrez Gene: 80174 Human Omim: 611661 Human SwissProt: Q8NFT6 Human Unigene: 369998 Human Unigene: 728986 Human |
| 產品圖片 |
Sample: Hela Cell (Human) Lysate at 30 ug
Primary: Anti-DBF4B (bs-5748R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18kD
Observed band size: 18kD
Sample: 293T Cell (Human) Lysate at 30 ug
Primary: Anti-DBF4B (bs-5748R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18kD
Observed band size: 18kD
Sample: U2os Cell (Human) Lysate at 30 ug
Primary: Anti-DBF4B (bs-5748R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18kD
Observed band size: 18kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
