| 產品編號 | bs-4134R |
| 英文名稱 | Rabbit Anti-MAP4K1 antibody |
| 中文名稱 | 造血祖細胞激酶1抗體 |
| 別 名 | Hematopoietic progenitor kinase 1; Hematopoietic progenitor kinase; HPK1; Human Hematopoietic Progenitor Kinase; M4K1_HUMAN; Map4k1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; Mitogen Activated Protein Kinase Kinase Kinase Kinase 1; Mitogen-activated protein kinase kinase kinase kinase 1. |
| 研究領域 | 免疫學 信號轉導 激酶和磷酸酶 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Cow) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 91kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAP4K1: 231-330/833 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Function: May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Subunit: Interacts with MAP3K1. Tissue Specificity: Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CNH domain. Contains 1 protein kinase domain. SWISS: Q92918 Gene ID: 11184 Database links: Entrez Gene: 11184 Human Entrez Gene: 26411 Mouse Omim: 601983 Human SwissProt: Q92918 Human SwissProt: P70218 Mouse Unigene: 95424 Human Unigene: 148278 Mouse |
| 產品圖片 |
Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti-MAP4K1 (bs-4134R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91 kD
Observed band size: 91 kD
Blank control: Hep G2 Cells(blue),fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Primary Antibody: Rabbit Anti-MAP4K1/AF647 Conjugated antibody (bs-4134R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
