| 產品編號 | bs-3888R |
| 英文名稱 | Rabbit Anti-NDUFS3 antibody |
| 中文名稱 | 線粒體復合物NDUFS3蛋白抗體 |
| 別 名 | CI 30KD; Complex I 30KD; NADH-Ubiquinone Oxidoreductase Fe-S Protein 3; NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; I-30kD; CI-30kD; NADH-ubiquinone oxidoreductase 30 kDa subunit. |
| 研究領域 | 腫瘤 細胞生物 免疫學 神經生物學 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30kDa |
| 細胞定位 | 細胞漿 細胞膜 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NDUFS3: 201-264/264 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The multisubunit NADH:ubiquinone oxidoreductase is the first enzyme complex in the electron transport chain of mitochondria. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction contains NDUFS1, NDUFS2, NDUFS3, NDUFS4, NDUFS5, NDUFS6, and NDUFA5. Subunit: Mammalian complex I is composed of 45 different subunits. Interacts with NDUFAF3. Subcellular Location: Mitochondrion inner membrane. Similarity: Belongs to the complex I 30 kDa subunit family. SWISS: O75489 Gene ID: 4722 Database links: Entrez Gene: 4722 Human Entrez Gene: 68349 Mouse Omim: 603846 Human SwissProt: O75489 Human SwissProt: Q9DCT2 Mouse Unigene: 502528 Human Unigene: 30113 Mouse |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NDUFS3) Polyclonal Antibody, Unconjugated (bs-3888R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NDUFS3 Polyclonal Antibody, Unconjugated(bs-3888R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
