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Rabbit Anti-IDH2  antibody (bs-3947R)  
~~~促銷代碼KT202411~~~
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產品編號 bs-3947R
英文名稱 Rabbit Anti-IDH2  antibody
中文名稱 線粒體異檸檬酸脫氫酶抗體
別    名 mitochondrial; ICD-M; IDH; IDH2; IDHP_HUMAN; IDP; Isocitrate dehydrogenase [NADP]; Isocitrate dehydrogenase [NADP], mitochondrial; mNADP-IDH; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase.  線粒體異檸檬酸脫氫酶(ICDHm);
Specific References  (1)     |     bs-3947R has been referenced in 1 publications.
[IF=6.126] Nanni S et al. Metabolic Reprogramming by Malat1 Depletion in Prostate CancerCancers (Basel).2020 Dec 22;13(1):E15.  WB ;  Human.  
研究領域 腫瘤  細胞生物  免疫學  染色質和核信號  轉錄調節因子  激酶和磷酸酶  新陳代謝  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Rat (predicted: Mouse,Dog,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=0.2ug/test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 51kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human IDH2: 251-350/452 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 IDH2 (isocitrate dehydrogenase 2 (NADP+), mitochondrial), also designated NADP+-specific ICDH; isocitrate dehydrogenase, mitochondrial; and oxalosuccinate decarboxylase, is a 452 amino acid enzyme encoded by the human gene IDH2. IDH2 belongs to the isocitrate and isopropylmalate dehydrogenases family and contains two nucleotide binding regions. IDH2 is involved in the reduction of NADP+ to NADPH and maintains the supply of glutathione (GSH) in mitochondria. It is believed to play a role in intermediary metabolism and energy production. IDH2 also tightly associates with the pyruvate dehydrogenase complex. IDH2 is found in the mitochondrion as a homodimer and can bind one magnesium or manganese ion per subunit.

Function:
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.

Subunit:
Homodimer.

Subcellular Location:
Cytoplasm. Peroxisome.

DISEASE:
Defects in IDH1 are involved in the development of glioma (GLM) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. Elevated levels of R(-)-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

Similarity:
Belongs to the isocitrate and isopropylmalate dehydrogenases family.

SWISS:
P48735

Gene ID:
3418

Database links:

Entrez Gene: 3418 Human

Entrez Gene: 269951 Mouse

Entrez Gene: 361596 Rat

Omim: 147650 Human

SwissProt: P48735 Human

SwissProt: P54071 Mouse

SwissProt: P56574 Rat

Unigene: 596461 Human

Unigene: 246432 Mouse

Unigene: 290925 Mouse

Unigene: 3490 Rat



IDH2又稱異檸檬酸脫氫酶,他有有兩種類型的酶:以NAD為輔酶的酶(EC1.1.1.41)和NADP的酶(EC1.1.1.42),兩者催化同一反應。在胞漿中以NADP為輔酶,在線粒體內膜以NAD為輔酶。    IDH2在三羧酸循環中正常起作用的是NAD為輔酶的酶,他是提供為ADP激活的變構酶。
產品圖片
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IDH2) Polyclonal Antibody, Unconjugated (bs-3947R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IDH2 Polyclonal Antibody, Unconjugated(bs-3947R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IDH2 Polyclonal Antibody, Unconjugated(bs-3947R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IDH2 Antibody(bs-3947R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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