| 產品編號 | bs-2652R |
| 英文名稱 | Rabbit Anti-SPHK1 antibody |
| 中文名稱 | 鞘氨醇激酶1抗體 |
| 別 名 | sphingosine kinase 1; SK 1; SK1; SPHK 1; SPHK; SPK; SPK 1; SPK1; Sphk1; SPHK1_HUMAN. |
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Specific References (9) | bs-2652R has been referenced in 9 publications.
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| 研究領域 | 腫瘤 細胞生物 信號轉導 細胞凋亡 激酶和磷酸酶 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Cow,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞核 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPHK1: 1-100/384 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Tissue Specificity: Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Similarity: Contains 1 DAGKc domain. SWISS: Q9NYA1 Gene ID: 8877 Database links: Entrez Gene: 8877 Human Entrez Gene: 20698 Mouse Omim: 603730 Human SwissProt: Q9NYA1 Human SwissProt: Q8CI15 Mouse Unigene: 68061 Human Unigene: 20944 Mouse Unigene: 18522 Rat SPHK1參與鞘氨醇代謝、G蛋白偶聯受體蛋白信號轉導途徑，有抗細胞凋亡的作用，可被蛋白激酶C激活，參與細胞內信號轉導級聯、鈣介導信號轉導、調控細胞生長遷移、調控血管生成及成纖維細胞增殖。 |
| 產品圖片 |
Sample: Heart (Mouse) Lysate at 30 ug
Primary: Anti- SPHK1 (bs-2652R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52kD
Sample:
Lane 1: Mouse Pancreas tissue lysates
Lane 2: Mouse Lung tissue lysates
Lane 3: Rat Kidney tissue lysates
Primary: Anti-SPHK1 (bs-2652R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42-52 kDa
Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK1) Polyclonal Antibody, Unconjugated (bs-2652R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
