| 產品編號 | bs-2646R |
| 英文名稱 | Rabbit Anti-LAG3 antibody |
| 中文名稱 | 淋巴細胞活化基因-3（CD223）抗體 |
| 別 名 | Lymphocyte activation gene 3 protein; Lymphocyte activation gene 3; CD223; FDC protein; LAG 3; LAG-3; LAG3_HUMAN. |
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Specific References (1) | bs-2646R has been referenced in 1 publications.
[IF=2.976] Chen Y et al. Antitumor effects of the silencing of programmed cell death ligand?1 in colorectal cancer via immunoregulation. (2018) Oncol.Rep. IHC ; Mouse.
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| 研究領域 | 細胞生物 免疫學 自然殺傷細胞 t-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Horse) |
| 產品應用 | Flow-Cyt=1μg /test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58kDa |
| 檢測分子量 | 70 |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LAG-3: 201-300/525 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008] Function: Involved in lymphocyte activation. Binds to HLA class-II antigens. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: On cell surface of activated NK and T-lymphocytes. Similarity: Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q5BK54 Gene ID: 3902 Database links: UniProtKB/Swiss-Prot: P18627.5 Entrez Gene: 3902 Human Omim: 153337 Human SwissProt: P18627 Human Unigene: 409523 Human |
| 產品圖片 |
Blank control:Ctll-2.
Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (bs-2646R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Ctll-2.
Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (bs-2646R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
