| 產品編號 | bs-2095R |
| 英文名稱 | Rabbit Anti-Integrin alpha 1 antibody |
| 中文名稱 | 整合素α1抗體 |
| 別 名 | CD 49a; CD49a; CD49 antigen-like family member A; CD49a; CD49a antigen; Integrin alpha-1; ITA1_HUMAN; Itga 1; ITGA1; Laminin and collagen receptor; Very late activation protein 1; VLA 1; VLA-1; VLA1. |
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Specific References (3) | bs-2095R has been referenced in 3 publications.
[IF=4.85] Yao Ding. et al. 1,25D/VDR inhibits pancreatic β cell ferroptosis by downregulating FOXO1 expression in diabetes mellitus. CELL SIGNAL. 2022 Dec;:110564 IF ; Rat.
[IF=2.81] Zhang, Rui, et al. "Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells." Stem Cells International 2015 (2015). WB ; Rat.
[IF=2.741] Cui, Xiyao. et al. TMT-based proteomic analysis reveals integrins involved in the synergistic infection of reticuloendotheliosis virus and avian leukosis virus subgroup J. BMC VET RES. Bmc Vet Res. 2022 Dec;18(1):1-8 WB ; Chicken.
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| 研究領域 | 細胞生物 免疫學 信號轉導 干細胞 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Cow,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg /test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 128kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Integrin alpha 1: 1001-1179/1179 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Alpha 1 integrin along with alpha 2, alpha L and alpha M has a unique inserted domain. Integrin alpha 1 is a receptor for laminin and collagen. The alpha1 subunit is also known as CD49a. CD49a associates with CD29 (beta 1 integrin), to form an alpha1 beta1 heterodimer, identified as the rat homologue to VLA1, which is involved in cellular adhesion to laminin and collagen. Function: Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Subunit: Heterodimer of an alpha and a beta subunit. Alpha-1 associates with beta-1. Interacts with RAB21. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain. SWISS: P56199 Gene ID: 3672 Database links: Entrez Gene: 3672 Human Entrez Gene: 109700 Mouse Omim: 192968 Human SwissProt: P56199 Human SwissProt: Q3V3R4 Mouse Unigene: 644352 Human Unigene: 482186 Mouse Unigene: 91044 Rat |
| 產品圖片 |
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: 293T (Human) Cell Lysate at 30 ug
Lane 3: U87MG (Human) Cell Lysate at 30 ug
Lane 4: HepG2 (Human) Cell Lysate at 30 ug
Lane 5: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-Integrin alpha 1 (bs-2095R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 150 kD
Observed band size: 160 kD
Blank control: U937(blue). Primary Antibody: Rabbit Anti- Integrin alpha 1 antibody(bs-2095R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-2095R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control: RSC96 cells(blue). Primary Antibody: Rabbit Anti-Integrin alpha 1 antibody(bs-2095R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
