| 產品編號 | bs-1747R |
| 英文名稱 | Rabbit Anti-Dopamine D5 receptor antibody |
| 中文名稱 | 多巴胺受體D5抗體 |
| 別 名 | D(1B) dopamine receptor; D(5) dopamine receptor; D1 beta dopamine receptor; D1B dopamine receptor; D1beta dopamine receptor; D5 dopamine receptor; DBDR; Dopamine receptor D1B; DRD 1B; DRD 5; DRD1B; DRD1L2; DRD5; MGC10601; DRD5_HUMAN; DRD5_RAT. |
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Specific References (6) | bs-1747R has been referenced in 6 publications.
[IF=11.685] Kristine Elisabeth Eberhard. et al. Neurotransmitter and Neurotransmitter Receptor Expression in the Saccule of the Human Vestibular System. Prog Neurobiol. 2022 Jan;:102238 IHC ; Human.
[IF=8.101] Linlin Meng. et al. Dopamine D1 receptor agonist alleviates acute lung injury via modulating inflammatory responses in macrophages and barrier function in airway epithelial cells. FREE RADICAL BIO MED. 2023 Jun;202:2 IHC ; Mouse.
[IF=4.522] Fujino S et al. Expression and function of dopamine in odontoblasts. J Cell Physiol. 2019 Oct 15. IHC-P&WB ; Rat.
[IF=4.181] Bai L et al. m6A demethylase FTO regulates dopaminergic neurotransmission deficits caused by arsenite.Toxicol Sci. 2018 Oct 1;165(2):431-446. WB ; Mouse.
[IF=2.35] Xu, Jiao-jiao, et al. "Dopamine D1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells." Acta Pharmacologica Sinica (2014). WB ; Human.
[IF=0] Hardt, Olaf, et al. "SSEA4 AND ST3GAL2 AS CHEMOTHERAPEUTIC DRUG RESPONSE BIOMARKERS." U.S. Patent No. 20,150,316,556. 5 Nov. 2015. FCM ; Mouse.
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| 研究領域 | 腫瘤 細胞生物 神經生物學 信號轉導 細胞膜受體 G蛋白偶聯受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg /test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat DRD5: 251-350/475 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. This receptor is neuron specific, localized primarily within limbic regions of the brain. Defects in DRD5 are a cause of blepharospasm and may be a cause of schizophrenia. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Neuron-specific, localized primarily within limbic regions of the brain. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P25115 Gene ID: 25195 Database links: Entrez Gene: 1816 Human Entrez Gene: 13492 Mouse Omim: 126453 Human SwissProt: P21918 Human SwissProt: Q8BLD9 Mouse Unigene: 380681 Human Unigene: 167154 Mouse Unigene: 138110 Rat
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| 產品圖片 |
Sample:
Brain (Mouse) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Primary: Anti- Dopamine D5 receptor (bs-1747R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53kD
Observed band size: 53kD
Sample:
Skin(Mouse) Lysate at 40 ug
Skin(Rat) Lysate at 40 ug
Primary: Anti-Dopamine D5 receptor (bs-1747R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Blank control: RSC96(blue). Primary Antibody:Rabbit Anti- DRD5 antibody(bs-1747R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Antibody (bs-1747R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1747R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
