| 產(chǎn)品編號 | bs-1567R |
| 英文名稱 | Rabbit Anti-RAMP1 antibody |
| 中文名稱 | 受體活性修飾蛋白1抗體 |
| 別 名 | calcitonin receptor like receptor activity modifying protein 1; RAMP 1; RAMP-1; CRLR activity modifying protein 1; receptor (G protein coupled) activity modifying protein 1; receptor activity modifying protein 1; Receptor activity modifying protein 1; RAMP1_HUMAN. |
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Specific References (2) | bs-1567R has been referenced in 2 publications.
[IF=2.605] Pauza AG et al. Alterations in enteric calcitonin gene-related peptide in patients with colonic diverticular disease. (2018) Autonomic Neuroscience.Sep 18. pii: S1566-0702(18)30176-0 IF ; Human.
[IF=1.86] Qiao, Xi, et al. "Intermedin is upregulated and attenuates renal fibrosis by inhibition of oxidative stress in rats with unilateral ureteral obstruction."Nephrology (2015). WB ; Rat.
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| 研究領(lǐng)域 | 細胞膜受體 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 14kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RAMP1: 118-148/148 <Cytoplasmic> |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the RAMP family of single transmembrane domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin receptor like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin gene related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein, CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface. Function: Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL. Subunit: Heterodimer of CALCRL and RAMP1. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed in many tissues including the uterus, bladder, brain, pancreas and gastro-intestinal tract. Similarity: Belongs to the RAMP family. SWISS: Q9WTJ5 Gene ID: 10267 Database links: Entrez Gene: 10267 Human Entrez Gene: 51801 Mouse Omim: 605153 Human SwissProt: O60894 Human SwissProt: Q9WTJ5 Mouse Unigene: 471783 Human Unigene: 3272 Mouse Unigene: 12265 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Recombinant human RAMP1 protein(bs-41350P)
Primary: Anti-RAMP1 (bs-1567R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: / kDa
Observed band size: 32 kDa
This image was generously provided by Xi Qiao from the Second Hospital of Shanxi Medical University. Formaldehyde fixed and paraffin embedded rat kidneys labeled with Rabbit Anti-RAMP1 Polyclonal Antibody (bs-1567R) and followed by conjugation to a secondary antibody and counterstained with hematoxylin.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RAMP1 Polyclonal Antibody, Unconjugated(bs-1567R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
