| 產(chǎn)品編號 | bs-0448R |
| 英文名稱 | Rabbit Anti-NIS antibody |
| 中文名稱 | 鈉碘轉(zhuǎn)運體蛋白抗體 |
| 別 名 | Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na+/I- cotransporter; Na+/I-symporter; NIS; SC5A5_HUMAN; SLC5A5; sodium iodide symporter; Sodium-iodide symporter; Sodium/iodide cotransporter; Solute carrier family 5 (sodium iodide symporter) member 5; Solute carrier family 5; Solute carrier family 5 member 5. |
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Specific References (11) | bs-0448R has been referenced in 11 publications.
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| 研究領(lǐng)域 | 免疫學(xué) 通道蛋白 轉(zhuǎn)運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NIS: 525-618/618 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
catalyzes Na+/I- symporter activity plays a role in iodide transport and thyroid hormone generation. Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane bound glycoprotein with 13 membrane spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid. Function: Mediates iodide uptake in the thyroid gland. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Expression is primarily in thyroid tissue, but also to a lower extent in mammary gland and ovary. Expression is reduced in tumors. DISEASE: Thyroid dyshormonogenesis 1 (TDH1) [MIM:274400]: A disorder characterized by the inability of the thyroid to maintain a concentration difference of readily exchangeable iodine between the plasma and the thyroid gland, leading to congenital hypothyroidism. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. SWISS: Q99PN0 Gene ID: 6528 Database links: Entrez Gene: 6528 Human Omim: 601843 Human SwissProt: Q92911 Human Unigene: 103983 Human Unigene: 584804 Human Unigene: 10505 Rat 鈉/碘轉(zhuǎn)運體蛋白-NIS，是位于甲狀腺細胞基底膜上的一類膜抗原，在甲狀腺對碘的主動轉(zhuǎn)運過程中發(fā)揮重要作用。 鈉/碘轉(zhuǎn)運蛋白受甲狀腺刺激素(TSH)、碘、細胞因子和生長因子等多種因素的廣泛調(diào)節(jié)。自身免疫性甲狀腺疾病時NIS的表達發(fā)生了明顯變化，患者體內(nèi)出現(xiàn)抗NIS自身抗體。 此外，NIS還與甲狀腺冷結(jié)節(jié)和先天性甲狀腺機能減退等甲狀腺疾病的發(fā)病機理密切相關(guān)，近年來得到了足夠的重視。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Stomach tissue lysates
Lane 2: Rat Stomach tissue lysates
Primary: Anti-NIS (bs-0448R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 48 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NIS Polyclonal Antibody, Unconjugated(bs-0448R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NIS Polyclonal Antibody, Unconjugated(bs-0448R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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