| 產品編號 | bs-0527R |
| 英文名稱 | Rabbit Anti-CD10 antibody |
| 中文名稱 | CD10抗體 |
| 別 名 | NEP_HUMAN; Neprilysin; MME; membrane metalloendopeptidase; NEP; SFE; CD10; CALLA; CMT2T; SCA43; Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; Neutral endopeptidase; Skin fibroblast elastase; |
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Specific References (3) | bs-0527R has been referenced in 3 publications.
[IF=3.439] Wang L et al. TGF-β1 stimulates epithelial–mesenchymal transition and cancer-associated myoepithelial cell during the progression from in situ to invasive breast cancer. Cancer Cell Int. 2019 Dec 19;19:343. ICF ; Human.
[IF=2.349] Yasuno Yamaguchi. et al. Hyperthyroidism exacerbates ischemic reperfusion injury in the kidney. 2021 Oct 09 FC ; Mouse.
[IF=1.966] Tian L et al. Gingerol inhibits cisplatin-induced acute and delayed emesis in rats and minks by regulating the central and peripheral 5-HT, SP, and DA systems. J Nat Med. 2019 Nov 25. IHC-P ; rat&mink.
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| 研究領域 | 腫瘤 細胞生物 免疫學 干細胞 細胞表面分子 細胞類型標志物 淋巴細胞 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=3μg /test,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 82kDa |
| 檢測分子量 | 100 |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD10: 490-530/750 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. [provided by RefSeq, Aug 2017] Function: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Post-translational modifications: Myristoylation is a determinant of membrane targeting. Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity. Similarity: Belongs to the peptidase M13 family. SWISS: P08473 Gene ID: 4311 Database links: Entrez Gene: 4311 Human Entrez Gene: 17380 Mouse Omim: 120520 Human SwissProt: P08473 Human SwissProt: Q61391 Mouse Unigene: 307734 Human Unigene: 296022 Mouse Unigene: 33598 Rat CD10即共同型急性淋巴細胞白血病抗原,是II型跨膜糖蛋白.CD10表達于早期B淋巴細胞,某些粒性白細胞,骨髓基質細胞,部分上皮組織及其起源的腫瘤細胞中也有表達.主要應用于某些惡性淋巴瘤和白血病的分型.CD10是一種94kD的鋅依賴性細胞膜金屬結合蛋白，參與細胞神經肽和激素肽的后分泌過程。它廣泛地分布于腎、肝、小腸、胎盤、脈絡膜叢、腦腺、腺皮質和白細胞，CD10是生發中心細胞及所致淋巴瘤的標志物。 |
| 產品圖片 |
Sample:
Lane 1: Rat Kidney tissue lysates
Lane 2: Human RAMOS cell lysates
Primary: Anti-CD10 (bs-0527R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 100 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD10) Polyclonal Antibody, Unconjugated (bs-0527R) at 1:25 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD10) Polyclonal Antibody, Unconjugated (bs-0527R) at 1:25 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD10) Polyclonal Antibody, Unconjugated (bs-0527R) at 1:25 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
