| 產品編號 | bs-0084R |
| 英文名稱 | Rabbit Anti-Caspase-6 subunit p18 antibody |
| 中文名稱 | 半胱胺酸蛋白酶蛋白-6抗體 |
| 別 名 | Caspase-6; Caspase-6 p18; cleaved Caspase-6 p18; Apoptosis Related Cysteine Protease; Apoptotic cysteine protease MCH2; Apoptotic protease MCH 2; Apoptotic protease MCH2; Casp 6; Casp6; Caspase 6 apoptosis related cysteine protease; Caspase 6 precursor; Caspase6; Human cysteine protease Mch2 isoform alpha; Mch 2; Mch2; CASP6_HUMAN; caspase-6 isoform alpha precursor. |
| 研究領域 | 細胞生物 神經生物學 信號轉導 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Caspase-6 subunit p18: 27-130/293 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in two transcript variants that encode different isoforms. [provided by RefSeq, Jul 2008] Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Subcellular Location: Cytoplasm. Post-translational modifications: Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits. Similarity: Belongs to the peptidase C14A family. SWISS: P55212 Gene ID: 839 Database links: Entrez Gene: 839 Human Entrez Gene: 12368 Mouse Omim: 601532 Human SwissProt: P55212 Human SwissProt: O08738 Mouse Unigene: 654616 Human Unigene: 281379 Mouse Unigene: 88160 Rat Caspase-6 與Caspase-3有38%的同源性，屬胱氨酸-天冬氨酸蛋白酶家族。在凋亡執行階段起中心作用，可被Caspase-7、-8、-10剪切。它是唯一一個可以剪切核纖層蛋白的Caspase。 |
| 產品圖片 |
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Caspase-6 subunit p18 (bs-0084R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18/33 kD
Observed band size: 33 kD
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Caspase-6 subunit p18 (bs-0084R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18/33 kD
Observed band size: 33 kD
Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Caspase-6 Polyclonal Antibody, Unconjugated(bs-0084R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caspase-6 Polyclonal Antibody, Unconjugated(bs-0084R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Tissue/cell: mouse intestine tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caspase-6 Polyclonal Antibody, Unconjugated(bs-0084R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
